Pre-laboratory 1. How are microorganisms destroyed by moist heat? By dry heat? 2
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Pre-laboratory 1. How are microorganisms destroyed by moist heat? By dry heat? 2. Are some microorganisms more resistant to heat than others? Why? 3. Is moist heat more effective than dry heat? Why? Why does dry heat require higher temperatures for longer time periods to sterilize than does moist heat? 4. 5. What is the relationship of time to temperature in heat sterilization? Explain. Would you recommend boiling or baking to sterilize a soiled surgical instrument? Why? 6. 7. What kinds of clean hospital materials would you sterilize by baking? Why?Explanation / Answer
1. Moist heat destroyes the microorganisms by denaturing the macromolecules within them, such as proteins. It also kills 'em by lysing their cell-wall/membranes.
whereas, dryheat destryoes microorganisms by drying the cells off and causing oxidative free radical damage. And in some procedures ut can burn the microorganisms to ashes.
2. Yes, some set of bacteria are more resistant to heat then other. Such set of bacteria are called thermophiles.
Heat generally destroyes the cells by denaturing proteins and other macromolecules. In this bacteria the enzymes and other proteins tend to have functionality at higher tempratures.
Besides this some spores of microorganisms have such structures that can negate the effects of higher tempratures, which may kill majority of the microbes.
3. Moist heat is more effective than dryheat because, the moist heat uses high pressured steam or wapour of water. And this high pressured wapour has significantly high penetrating power than dry-heat.
4. Dry heat uses Coduction of heat for the sterilization process. In which the outer layer absorbs the heat and the passes it to under lying layer or surface. When the temprature is homogenised through all the layer the sterilization process starts. Thus making it an longer process to carry out.
Whereas in moist heat, pressurized steam or wapour of water has higher penetrating power thus it will make this procedure a lot more quicker.
5. Generally time and temprature for sterilization are in inverse proportion to each other.
Which is an obvious because the higher the temprature the less time it will take to destroy all the microbes. And vica-versa if the temprature is kept lower, the higher time will be needed for killing all the microbes.
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