Exercise 3 - Biology 105 Estimating the Size of Cells Using a Compound Light Mic

Question

Exercise 3 - Biology 105 Estimating the Size of Cells Using a Compound Light Microscope Objectives of this lab are toc 1. Learn how to use the compound light microscope. Lcarm how to make a preparation for viewing on a slide. 2. 4. 5. 6. Make scale drawings. Estimate the lengths of cells seen with the microscope. Draw a bar graph comparing the lengths of the various cells you measure One principle of biology is that all organisms are celbular. That is, they are made up of one more cells. Cells live in a wide varicty of habitats and perform a wide variety of functions. This is reflected by a multitude of cell shapes and sizes. Today you will observe several different kinds of cells draw them, and estimate their lengths The scopes you will use are compound light microscopes. Since they have two oculars they are called binn called binocular microscopes. I. Determine the Total Magnification with each objective lens Each lens (both obiectives and oculars) has a particular magnifying power. The ocular lens has a Each objective lens has a number of a particular color. These numbers are number followed by an X. the magnifying power for that lens. Total magnification of the microscope is found by multiplying the power of the ocular lens by the power of the objective lens. Use Table 1 to record your results as you calculate the total magnification obtained with each objective lens. Table 1: Parameters of a compound light microscope used in Principles of Biology Power of Ocular Power of Objective Total Magnification Diameter of Field of Lens Lens View (mm) l0 X 10 x I to record your results. The field of view is the circular area that one sees as one looks through the microscope. To determine the diameter of the field of view, place a plastic ruler under the low power (4 x) objective Focus on the ruler and lay it so it crosses the diameter of the field of view. Record the diameter of the field of view in millimeters in Table 1. Record the diameter of the field of view for the 10x objective lens

Explanation / Answer

as the objective lens power increases the field of view decreases. resolution increases.

power of ocular lens power of objective lens total magnification diameter of field of view 10x 4x 40x 3 mm 10x 10x 100x 2 mm 10x 40x 400x 0.3 mm

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