please be specific. 6a. (5 pts) You identified a biosynthetic operon that is req
ID: 206021 • Letter: P
Question
please be specific.
6a. (5 pts) You identified a biosynthetic operon that is required to synthesize serine in your favorite bacterium Your first experiments identified a repressor - SerR - that blocks transcription of this operon when it is bound to serine. But you also find that transcription is still repressed in the presence of serine when the repressor is deleted from the genome. You therefore hypothesize that attenuation also plays a role in regulation. What sequence would you predict to find in this operon if you are correct? 6b. (5 pts) What additional mutation would you add to your serR null mutant to test your prediction directly?Explanation / Answer
A. The sequence rich in GC and followed by U
The base-pair to form a G=C rich stem and loop structure followed by a series of uridylate residues, a structure that resembles a transcription terminator.
B. GC rich region is mtated in the genes to AT it leads to inhibition of attenuation. In presence of serine the transcription take place due to ser R null mutation and no fromation of attenuation. So we conclude the attenuation is important in termiantion process.
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