1. What base(s) is(are) present at the 3\'-end nucleotide of the molecules in ba
ID: 205976 • Letter: 1
Question
1. What base(s) is(are) present at the 3'-end nucleotide of the molecules in band a?
A Adenine B Guanine C Thymine D Uracil E Cytosine F Adenine or Thymine G Adinine, Thymine, Guanine, or Cytosine
2. Why can you see the bands in Figure 3B?
A The proteins have been stained with Coomasie blue.
B The DNA fluoresces when stained with ethidium bromide.
C. A fluorescent tag was attached to dNTP's used as substrate for DNA Polymerase. The incorporated fluorescent nucleotides can be seen when viewed under UV light.
D The hyperchromic shift allow detection of single strand DNA in the polyacrylamide gel
E Autoradiography
The catalytic properties of calf thymus DNA polymerase (Pol alpha) and the epsilon subunit of Escherichia coli DNA polymerase III were studied in an in vitro system. The reaction mixture contained the radioactively labeled synthetic DNA duplex shown in Fig. 3A. Epsilon subunit was added, incubation was performed at 30 °C, and samples were withdrawn from the mixture at the time points indicated in Fig. 3B. Each sample was divided into two aliquots, and the four deoxyribonucleoside triphosphates (dATP, dTTP, dGTP, and dCTP) were added and further incubated for 90 s in the absence (-Pol alpha) or presence (+Pol alpha) of DNA polymerase epsilon. The samples were electrophoresed in a denaturing polyacrylamide gel followed by autoradiography. Please remember that you should practice answering based on what you can substantiate rather than answering what you think that I think that I have thought is the right answer They are double-stranded DNA molecules. A. 16 nucleotides GGATGTTTC 30 nucleotides B. 0 1 2 4 8 121 6 0 1 2 | 4 8 1216 c subunit (min) Polo + Polo. DNA polymerase 123 4 5 67 8 91011121314 SampleExplanation / Answer
Adenine, Guanine,..... Here the Adenine is replaced by Thymine. And Guanine is replaced by Cytosine.
So answer is G
2)In DNA electrophoresis The loading gel is used for the DNA markers. It is used for the visual tracking of DNA migration. After the proper staining with the gel the bands can be see in the UV light.
So answer is B.
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