1. elow you have the seguence of both strand f rtiolar DNA segnent 5\' T T C A T
ID: 205026 • Letter: 1
Question
1. elow you have the seguence of both strand f rtiolar DNA segnent 5' T T C A T A A A A C C T A3 3' A A G T A T T T T G G A T 5' So, let's say that you decided to artificially replicate this particular segment in the lab (this is totally a thing that scientists do; more on that in the next unit) in a solution containing radioactively labeled guanine nucleotides. a) Will both daughter strands be radioactive? Explain your choice b) What if you replicated this segment in a solution containing radioactively labeled adenine nucleotides? Would both daughter strands be radioactive? Explain.Explanation / Answer
Explanation:
Question here asked is about the DNA replication. Semi-conservative nature of replication process resulted in two DNA strands each of them contain one parental strand. The newly synthesized is synthesized according to nucleotide sequence occurs in template strand. It is proven by Meselson and Stahl late in 1958 using radio label 15N isotope.
Here in give question
5’- T T C A T A A A A C T A -3’
3’- A A G T A T T T T G A T-5’
After replication strand will be as bellow ( Bold is newly synthesized)
5’- T T C A T A A A A C T A -3’
3’- A A G T A T T T T G A T-5’
5’- T T C A T A A A A C T A -3’
3’- A A G T A T T T T G A T-5’
If replication is processed in presence of radioactively labeled Guanine than newly synthesized strand will take up this radio labeled guanine and become radioactive. However parent strand remain un-radio-labeled.
Answer
Reason: In both the daughter strand one will be parental strand ( Not radio-active) and another is newly synthesized strand (radio labeled). On searching for radio activity, due to presence of radio-active guanine in both the daughter strand, they will found to be radioactive.
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