Why happens to primers in the forward and reverse direction during PCR? Select o

Question

Why happens to primers in the forward and reverse direction during PCR? Select one: O a. Only the forward primer, but not the reverse primer, hybridize with the template DNA O b. Only the reverse primer, but not the forward primer, hybridize O c. Both the forward and reverse primers hybridize to O d. The forward primer hybridizes to the complementary reverse O e. Double-stranded template DNA hybridizes with the forward and with the template DNA complementary regions of single-stranded template DNA primer reverse primers

Explanation / Answer

Option e is the correct answer. To perform a PCR, there is a need to enhance both strands, so there is a need a primer for one strand, called the forward primer, which is the beginning of the gene, and an other primer that will begin the complementary strand (in the 5' end), it's called the reverse primer.The forward primer attaches to the start codon of the template DNA (the sense strand), while the reverse primer attaches to the stop codon of the complementary strand of DNA (the anti-sense strand).

Related Questions

Navigate

• Browse (All)
• Browse W
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.