With respect to the Eosin Methylene Blue (EMB) agar, if an organism has dark pur

Question

With respect to the Eosin Methylene Blue (EMB) agar, if an organism has dark purple growth with a metallic sheen, what can we interpret from these results? How would you know if DNase activity is present when reading the results for the DNase Agar test? 5. 6. If you see complete clearing around the bacterial growth on a Blood agar plate what type of hemolysis could it be and name an organism that can produce this type of hemolysis? What can we find out from the Phenol Red broths with respect to the biochemical properties of bacteria? 7. Write the chemical equation for the breakdown of hydrogen peroxide (H202) by the enzyme catalase? 8. If you read the results for the Oxidase test after five minutes, what type of results would you get from oxidase positive and negative bacteria on the DrySlide card? 9. 10. What enzyme must bacteria have in order to utilize the citrate in the Simmons Citrate slants that you inoculated? What does this enzyme convert the citrate to? 11. Write the chemical equation for the hydrolysis of urea by the enzyme urease. What color will the Urea agar turn if organisms are positive for this test and why? 12. Based on the interpretation of the Triple Sugar Indole (TSI) agar slants, if you have a tube with a red slant and butt color after incubation, what can you infer from these results about the biochemical characteristics of your organism? 13. Why is tetrazolium salt added to the Motility test media? 14. With respect to the Enterotube Il coding system, if your organism had and ID code of 33643, which compartments would have a positive result? List the name of each compartment that is positive GENERAL MICROBIOLOGY LABORATORY MANUAL 50

Explanation / Answer

Q13) Tetrazolium indicates where bacterial growth has occurred. tetrazolium salt added to enhance visualization of bacterial growth.

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