, Hon do se break the hydrogem bonds holding double stranded DXA together during
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Question
, Hon do se break the hydrogem bonds holding double stranded DXA together during PCR? (1 point) 2 What is the purpose of the mortar and pestle in today's experiment? (1 point 3. The 5 end of DNA must react with the bond. (1 point) end of DNA to form a phosphodiester 4. Today we are testing com chips for- Point Mutations _ using PCR. (1 point) A. B. Enzyme Activity BT Transgene D. DNA replication 5. Why is it important not to contaminate the PCR tubes with anything besides the DNA we are looking to analyze? (1 point)Explanation / Answer
High temperature (94C ) is used to break the double stranded DNA hydrogen bond in PCR. Mortals and pestles used to break the tissues in a fine homogenous power (in case of seeds)/ liquid (in case of animal tissue like liver). It will breaks the disulphide bonds of cell surface proteins which binds tissue. in case of maize chips it will makes a fine powder of maize chips. The 5’ end of DNA must react with 3’ end of DNA to for a phosphodiester bond Corn chips PCR for BT transgene If we are contaminates PCR tube with other material there may be DNasase which will destroy our DNA sample or any other foreign DNA (may be bacterial, viral or other DNA) which hinder our PCR reaction and gives inappropriate result.
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