Problem 24.14 Constants Periodic Table Part A s RNA polymerase saturated with su
ID: 202145 • Letter: P
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Problem 24.14 Constants Periodic Table Part A s RNA polymerase saturated with substrates in vivo? It's saturated It's not saturated. Previous Answers Correct Part B Describe experiments that might indicate whether RNA polymerase is operating at Vmax with respect to its nucleotide substrates Check all that apply. Add aminoglycosides to a bacterial or cell culture and determine whether chain growth rates are increased and the protein pool sizes. Introduce the genes for the mutant purine and pyrimidine nucleotide synthesis enzymes into bacteria, and determine whether RNA chain growth rates are increased Add deoxynucleotides to a bacterial or cell culture and determine whether the dNTP pool sizes and chain growth rates are increased Introduce the genes for the mutant RNA polymerase into bacteria, ascertain that the rNTP pools are enhanced, and determine whether RNA chain growth rates are decreased. Add ribonucleosides to a bacterial or cell culture and determine whether the rNTP pool sizes and chain growth rates are increased Submit Request AnswerExplanation / Answer
Adding ribonucleotides into the culture medium if does not change in chaingrowth rates and rNTP pool then the enzyme is performing at Vmax. Hence option 5th is correct.
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