1.) Create a prokaryote gene at least 30 BP long. Mark the 5\' and 3\' ends. a.)
ID: 20097 • Letter: 1
Question
1.) Create a prokaryote gene at least 30 BP long. Mark the 5' and 3' ends.a.) Describe the function of this gene's product. Be creative!
2.) Describe the regulatory mechanism(s) that will govern the transcription of the gene. (don't make up)
3.) Initiate protein synthesis by transcribing the DNA template.
4.) Draw the tRNA molecules that will be used in the translation of the gene. "stick figures acceptable"
5.) Indicate the primary structure of the protein translated form the mRNA.
6.) Create a point mutation in the gene that will result in genotype change, but not a phenotype change. Circle this point mutation on the DNA strand. NAme an agent that causes point mutations.
7.) Create a point mutation in the gene that will result in a genotype and a phenotype change. Circle this point mutation on the DNA strand and describe the phenotype change.
8.) Create a frameshift mutation and indicate the effect the mutation will have on the primary structure of the protein. Name an agent that causes frameshift mutations.
9.) Irradiate the DNA with UV light and show the effects. Explain the effect the irradiation will have on transcription and how the damage could be repaired.
10.) Suppose this gene was located on a plasmid. Describe how it would be transferred to another bacterial cell via conjugation if the recipient was an F- cell and the donor was:
a.) An F+ cell
b.) An Hfr cell
c.) An F' cell
*Also indicate if the chromosomal genes could be included in the transfer.
11.) What is a blunt end?
12.) What is a sticky end?
13.) Describe, in detail, how you would insert the gene (or part of it) into a bacterial cell via a recombinant DNA process.
14.) Describe how you will determine if the recombination attempt was successful.
Explanation / Answer
ads
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.