I dont quite understand this question. Could you help me? -Mention a specific te

Question

I dont quite understand this question. Could you help me?

-Mention a specific technique with the application:

a) Purification of different charged proteins

b) Separation of proteins in electric field on the basis of isoelectric point

c) Determination if purity of protein purification

d) Purification of 180 kDa and 60kDa proteins

e) Separation of proteins in electric field on the basis of molecular weight and isoelectric

points

f) Separation of proteins in electric field on the basis of molecular weight

g) Technique used to separate two proteins that comigrate on SDS polyacrylamide gel

Explanation / Answer

The technique is Paper electrophoresis

The technique is used for the determination of isoelectric point, in the differential diagnosis of diseases in which there are abnormal hemoglobins, assay of pharmaceutical products and many more.

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