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CHEM 3801-01-Spring 2018 Instructor Dr Mandy Prasad Ph of Chemistry and Forensie

ID: 200231 • Letter: C

Question

CHEM 3801-01-Spring 2018 Instructor Dr Mandy Prasad Ph of Chemistry and Forensie Total Poists Total pts Received 20 Student Name: Dated: 02/22/2018 CHEM3801-01: Biochemistry Spring 2018 Homework Assignment-4A (Chapter-4) 27 Question 1. The primary structure of a protein is known and the location of internal disulfide bonds has been determined. How could one predict whether Print Received there might be secondary structures in my protein once it folds into a native conformation? A. Perform X-ray diffraction on my protein B. Perform a Ramachandran plot/analysis on my protein C. Run two-dimensional gel electrophoresis on my protein D. Determine if there is more than one polypeptide in the protein E. Identify specific motifs in the primary structure Answer: Question 2. A motif in the glycolysis enzyme hexokinase could contain all of the following, except: Poin Received A. beta-turn. B. beta-sheet. C. alpha-helix. D. disulfide bonds. E. All of the above could be found in a motif. Answer: Question 3. Which of the following statements about aromatic amino acids is Poin:2 correct? Received A) All are strongly hydrophilic. B) Histidine's ring structure results in its being categorized as aromatic or basic, depending on pH. c) On a molar basis, tryptophan absorbs more ultraviolet light than tyrosine. D) The major contribution to the characteristic absorption of light at 280 nm by proteins is the phenylalanine R group. E) The presence of a ring structure in its R group determines whether or not an amino acid is aromatic. Answer: Question 4. What important function do molecular chaperones perform? Point: A. Assemble protein subunits into a quaternary structure B. Help fold proteins into a native conformation C. Transport proteins to either the plasma membrane or release them from cells to Received the extracellular space D. Add cofactors, coenzymes or prosthetic groups to proteins as they are synthesized E. Move proteins from endoplasmic reticulum to the Golgi apparatus Answer: Page 1 of 3

Explanation / Answer

1. Answer: Perform a Ramachandran plot/analysis on my protein

2. Answer: E. All of the above can be found in a motif.

3. Answer: C. On a molar basis, tryptophan absorbs more ultraviolet light than tyrosine.

4. Answer: B. Help fold proteins into a native conformation

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