2. DNA replication rates Assuming that Figure 3.35 is a representative sample of

Question

2. DNA replication rates Assuming that Figure 3.35 is a representative sample of the replication process replication forks (a) (10 points) Using the scale bar, estimate the fraction of the total fly DNA shown in the micrograph. Note that the fly DNA is about 1.8 x 10* nucleotide pairs in size (b) (10 points) Based on the answer of (a) and the textbook content about the replication forks, estimate the number of DNA polymerase molecules in a eukaryotic cell like this one from the fly D. melanogaster (c) (10 points) There are eight forks in the micrograph. Estimate the lengths of the DNA strands between replication forks 4 and 5, counting up from the bottom of the figure. If a replication fork moves at a speed of roughly 40 bp/s, how long will it take for forks 4 and 5 to collide? direction of fork movement (d) (10 points) Given the mean spacing of the bubbles, estimate how long it will take to replicate the entire fly genome. 0.1 m Figure 3.35 Physical Biology of the Cell, 2ed. (O Garland Science 2013)

Explanation / Answer

1 nucleotide or a base is 3.4 Angstrom which is 3.4 x 10-4 that means total length of a fly DNA is 3.4 x10-4 * 1.8x108= 6.12 x 104 micro metre;

0.1/6.12 x 104

= 0.0163x 104

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