I am a cancer researcher. I have an antibody that recognizes the extracellular p

Question

I am a cancer researcher. I have an antibody that recognizes the extracellular portion of Notch and I know activated Notch is implicated in many types of tumors I want to detect for a given tumor wheth. cells of the same type. Based upon what you kniow which of the following statements could be true? Ineed to use SDS and beta-mercaptoethanol Tumor cells where Notch is activated should contain 1 Notch protein that I can see using my entit Tumor cells where Notch is activated should contain 2 Notch protein that can see using my ante should blot the gel I must use my antibody to see the Notch protein Tumor cells where Notch is activated should contain 3 Notch protein that I can see using my antib I need to separate out proteins based upon size I need to separate out proteins based upon charge I could perform PAGE for analysis I could perform agarose gel electrophoresis for analysis could make a 2D gel for analysis Click Save and submit to save and submit. Chick Save All Anisuwers to save all answers.

Explanation / Answer

You must use the antibody to see the notch protein...tumor cells where notch is activate should contain 1 notch protein that u can see using your antibody...then you should detect the protein-antibody complex based on their size...

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