I am a cancer researcher. I have an antibody that recognizes the extracellular p
ID: 199810 • Letter: I
Question
I am a cancer researcher. I have an antibody that recognizes the extracellular portion of Notch and I have conjugated it to a light producing chromophore. I know activated Notch is implicated in many types of tumors. I want to detect for a given tumor whether Notch activity has changed in comparison to wild type cells of the same type. Based upon what you know, which of the following statements could be true? (Hint: Use the information from the other qustions.) Tumor cells where Notch is activated should contain 2 Notch protein that I can see using my antibody. Tumor cells where Notch is activated should contain 1 Notch protein that I can see using my antibody. I could perform PAGE for analysis. OI could make a 2D gel for analysis. Tumor cells where Notch is activated should contain 3 Notch protein that I can see using my antibody. could perform agarose gel electrophoresis for analysis. I should blot the gel. I need to separate out proteins based upon size. I need to use SDS and beta-mercaptoethanol I need to separate out proteins based upon charge. OI must use my antibody to see the Notch protein.Explanation / Answer
The things you can do to sort out this proprob is
1) you should blot the gel
2) you can also separate the protein on the basis of the size..
3) you can also observe it through the light detection given by the flourophore..
4) the notch will activate two notch proteinpon the surface .!!
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