Question 6: More on PCR,5 points Imagine you are working with an old sample of D

Question

Question 6: More on PCR,5 points Imagine you are working with an old sample of DNA in which all the copies but one dsD NA molecule have degraded. You decide to try PCR anyway, to amplify the 500 base pair limb enhancer of the sonic hedgehog gene. A. 1 points. After 30 rounds of PCR, how many molecules of dsDNA will you have. Please round to the nearest billion. bilio n B. 2 point. What is the minimum number of copies of the top primer that you would need for 30 rounds of PCR? C. 2 point What is the minimum number of copies of the bottom primer that you would need for 30 rounds of PCR?

Explanation / Answer

1. If we start with a single molecule of DNA, after 30 cycles of PCR we would have 230 copies of DNA. so 230 = 1.02 x 109 which slightly more than one billion. Hence it will produce one billion DNA sequences.

2. The number of forward primers used will also be the same as number of DNA molecules formed after 30 cycles, because for each plus strand synthesis one forward primer is incorporated into the newly synthesized strand.

3. The number of reverse primers will also be equal to the number of amplicons produced as similar to forward primer, the reverse primer will be incorporated into the minus strand syntheis.

Related Questions

Navigate

• Browse (All)
• Browse Q
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.