Question 6: More on PCR,5 points Imagine you are working with an old sample of D

Question

Question 6: More on PCR,5 points Imagine you are working with an old sample of DNA in which all the copies but one dsDNA molecule have degraded. You decide to try PCR anyway, to amplify the 500 base pair limb enhancer of the sonic hedgehog gene. A. 1 points. After 30 rounds of PCR, how many molecules of dsDNA will you have. Please round to the nearest billion. B. 2 point. What is the minimum number of copies of the top primer that you would need for 30 rounds of PCR? C. 2 point. What is the minimum number of copies of the bottom primer that you would need for 30 rounds of PCR?

Explanation / Answer

after 30 cycles, you would have an approximately 1100000000 number of copies, 1073741824 to be exact.

you would require 536870912 number of copies of the primer fir 30 rounds. (this is same for both top and bottom primers.)

In case you have any further query, feel free to leave a comment down below. Thank you.

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