Table 2: 260/280 Ratio Values for 4 Samples of DNA Sample 260/280 ratio · 6875 1
ID: 199612 • Letter: T
Question
Table 2: 260/280 Ratio Values for 4 Samples of DNA Sample 260/280 ratio · 6875 1,6262 . 960 3 Based on these ratios, how pure are the DNA samples you were given? If a DNA sample is not pure, what are the possible contaminants of the sample? 5. a) b) 6. For Sample 1, determine the concentration of the DNA in solution assuming that you are dealing with pure dsDNA. The dilution factor is 10. 1.0 A260 Unit of double-stranded DNA 50 g/mL 1.0 A260 Unit of single-stranded DNA 37 g/mL 1.0 A260 Unit of RNA 40 g/mL QuestionsExplanation / Answer
Answer A) sample 2 is dna
sample 4 is RNA
sample 1,3 have either very low concentration of DNA or they are contaminated.
Answer B) Abnormal 260/280 ratios usually indicate that a sample is contaminated by residual phenol, guanidine, or other reagent used in the extraction protocol, in which case the ratio is normally low. Inaccurate ratios may also be encountered at very low concentrations (< 10 ng/µl) of nucleic acids.
For the second question, i believe we would need seperate value for absorbance a 260 for samples rather than the ratio of 260 and 280.
If i can help you any further, please let me know in the comment box. Thank you.
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