The parameters for this enzyme are: Km = 40 M Vmax = 3 pmol/min Calculate the in

Question

The parameters for this enzyme are: Km = 40 M Vmax = 3 pmol/min Calculate the initial reaction velocity (v) of Bcr-Abl-catalyzed reactions when protein substrate (s) is present at the following concentrations. Show your work. *Your answers must have units of pmol/min.* • [s] = 0.00001 M The parameters for this enzyme are: Km = 40 M Vmax = 3 pmol/min Calculate the initial reaction velocity (v) of Bcr-Abl-catalyzed reactions when protein substrate (s) is present at the following concentrations. Show your work. *Your answers must have units of pmol/min.* • [s] = 0.00001 M The parameters for this enzyme are: Km = 40 M Vmax = 3 pmol/min Calculate the initial reaction velocity (v) of Bcr-Abl-catalyzed reactions when protein substrate (s) is present at the following concentrations. Show your work. *Your answers must have units of pmol/min.* • [s] = 0.00001 M

Explanation / Answer

Initial velocity can be calculated by using the formula: V0 = Vmax ( [s]/ [s] + Km). Where V0 = initial velocity, [s] = substrate concentration and Vmax = maximum velocity.

Thus, V0 = 3 ( 10/ 10 + 40000000) = 3 (10/ 40000010) = 7.45 X 10-7

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