REVIEW QUESTIONS 1. Where in or on the human body would you expect to find Staph
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REVIEW QUESTIONS 1. Where in or on the human body would you expect to find Staphylococcus epidermidis? hich one of the three organisms used in this exercise is most likely to cause disease? 3. What enzyme causes bubbles to form when cells are exposed to hydrogen peroxide? What is the chemical formula of the gas that produces the bubbles? 4. Did any of the colonies on your mannitol salt agar plate inoculated with a skin swab resemble any of the known organisms? If so, what organism(s) do you think you found and what clues do you have? . How could you distinguish between S, aureus and S epidermidis using the differential and selective media employed in this exercise?Explanation / Answer
1. Staphylococcus epidermidis is the part of human flora and mostly present on skin and less coman in mucosal layer.
2. Here you have not mentioned the three organisms but among two organisms (S. aureus and S. epidermidis) which are present in this section, S. aureus is highly infectious and has a vast range from superficial to systemic illnesses including intoxications.
3. Catalase enzyme releases from broken cell and decompose hydrogen peroxide. Due to decomposition of hydrogen perxide, oxygen gas releases which forms bubbles. The chemical formula of oxygen is O2.
4. Since 40 - 50% of healthy human skin contain staphylococcus species, there is a chance of finding clonoies of staphylococcus species in mannitol salt agar plate. The salt concentration of mannitol agar plate inhibit the growth of other pathogenic species but tolerated by staphylococcus aureus (yellow colonies) and staphylococcus epidermidis (colouless to pink colonies) and other species of staphylococcii. Micrococcus species may also be there because of part of skin flora.
5. Here mannitol salt agar is used as selective and differential media for distinguishing S. aureus and S. epidermidis. S. aureus forms yellow colonies while S. epidermidis forms colourless to pink colonies.
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