REVIEW QUESTIONS 1. What is the difference between a pure and a mixed culture? 2
ID: 195763 • Letter: R
Question
REVIEW QUESTIONS 1. What is the difference between a pure and a mixed culture? 2. Could you see separated purple, yellow and white colonies on the streak or loop dilution pour plates? Which pour plate gave the best separation of all the three bacterial species? hich organism produced which color of pigment? 4. Which technique, the streak plate or the loop dilution pour plate, would you prefer to use if someone 5. After obtaining individual colonies from a mixed culture on a streak or pour plate, what would you do 6. Why are you required to wash down your bench top with disinfectant before you begin and after each gave you a mixed culture and asked you to isolate the individual organisms? Why? next if you wanted to grow only one of the organisms as a pure culture in a tube of broth? lab?Explanation / Answer
1. It is culture of microbes containing more than one type of microorganism or strains of microorganisms is called mixed culture and culture containing only one strain is called pure culture.
2. Colonies can be separated by streaking method because in pour plate colonies are entrapped inside the media.
3. Organism synthesize different type of pigments according to their genetic makeup.
4. Individual microorganisms can be isolated by strak plate method because in streaking cells are diluted to such a extent that these can easily isolated.
5.for obtaining pure culture from mixed culture microbes are inoculated in broth medium with the help of sterilized inocula
ting loop and then incubated for growth .
6 disinfection is performed to decreasing microorgamnism concentration on bench top.
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