Website Link: http://www.addgene.org/browse/sequence_vdb/1979/ DIGEST TAB Questi

Question

Website Link: http://www.addgene.org/browse/sequence_vdb/1979/

DIGEST TAB

Questions: I posted the link to lead to the digest tab (to the right of the link)

1) How many unique restriction enzymes are present that would cut the plasmit only once?

2) How many restrictions enzymes have 2 recognition sites? Name them

acggattcac cacuoCa 1301 1351 gtgaatgcge 1401ctccagcagc 145 gggtgogcat aaaccaaccc cgcacgcggc gatogtgctc ttggcagaac gcatetcggg ctgtogttga atatccatcg cagegttggg ggacceggct 1501 ap and FeaturesSequence Blast Align Digest Translate E5 Cutters Linear Digest Circular Digest pBR322: 4361 bp Clal (24) Hindii (29) Bmtl (233) EcoRV (1 Nhel (229) BamHl (37e) SgrAl (410) SPA ISG6) ell (651) BspEl (1664 Pvull (2066) Asel (3538) Psti (3611) Sal Eagl (939) Nrul (974 Mscl (1446) Bs12171 (2246) Ndel (2296 Pul (2473) vu (3736 al (3846 Sspl (4170) Aatll (4288)

Explanation / Answer

A- Unique Cutter - 15 enzymes

ClaI, HindIII, EcoRV, BamHI, NheI, SalI, EagI, NruI, MscI, NdeI, PstI, AseI, EcoRI, AatII, PvuI
B- Double cutter 16 enzymes
AccI, AcuI, BanHI, BciVI, BseYI, BsrBI, BsrDI, BstAPI, BtgI, BtsI, DrdI, EarI, HincIII, PflMI, PpuMI, XmnI

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