to test GMO status of foods (6 points total, 2 points per question). This gel be
ID: 198158 • Letter: T
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to test GMO status of foods (6 points total, 2 points per question). This gel below illustrates the result Hemember that the CaMV plant master mix with a band at s that a Biol 324 student obtained in the GMO PCR lab exercise. promoter and the NOS terminator are amplified using the GMO master mix s expected at 200 bps, while the Photosystem 2 (PS2) gene is amplified using the 500 bp. The non-GMO control was prepared by the student from oats certified to be non-GMO; the G MO positive control DNA was provided as pure DNA, and served as a M: DNA size marker Lane 1: Oats; GMO MM Lane 2: Oats; plant MM Lane 3: Sugar peas (test food #1): GMO MM Lane 4: Sugar peas; plant MM Lane 5: Bean sprouts (test food #2); GMO MM Lane 6: Bean sprouts; Plant MM Lane 7: GMO (+) control; GMO MM Lane 8: GMO (+) control: Plant MM M 1 2 3 4M567 8 M 1000bp -750bp 400bp 300bp 200bp Oats was used as a negative control. If this control were omitted, the student would not be able to detect one type of potential problem in this PCR experiment. What problem is that? a. True or False: We can conclude from these data that the student failed to extract DNA from test food 1. Explain your choice. b. True or False: We can conclude from these data that the CaMV/NOS primers are degraded or defective in some way. Explain your choice. c.Explanation / Answer
5a.If the negative control was not used, we would not be able to say for sure if the positive results obtained in lanes 3 and 5 were false positives arising from factors such as contamination in the GMO MM.
b.False. The student was able to extract DNA from test food 1 as evident by the presence of a 200bp amplification band in lane 3.
c. False. The fact that the expected 200bp amplification bands have been obtained in lanes 3,5 and 7 and not in lane 1 with the GMO MM shows that the CaMV/NOS primers are not degraded in any way.
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