2 35 QUESTION 5 Suppose you set up the PCR cycle and run the PCR product in agar

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2 35 QUESTION 5 Suppose you set up the PCR cycle and run the PCR product in agarose gel electrophoresis. You discovered that there ae multiple bands of different sizes. What could be the reason for that? @ A The denaturation temperature is too low e B. The annealing temperature is too low O C The annealing temperature is too high D The extension temperature is too high QUESTION 6 Which of the following is NOT common between eukaryotic and prokaryotic DNA replication? A Semi conservative mode of DNA replication e B. Bidirectional @ C Have both leading and lagging strands D. Use DNA primers QUESTION 7 Click Sque and Submit to save and submit. Chick Save All Answers to save all ansuers.

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5. B.Anneling temperature is too low.

If the temperature is too high, no annealing occurs, but if it is too low, non-specific annealing will increase dramatically. Due to non specific annealing it binds in different region and gives multiple DNA strand.

6. D. Use DNA primer

In replication RNA priner is involved. RNA primer are synthesized by specialized enzymes called primases.

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