1. Which of the following answers is (are) true about size exclusion chromatogra
ID: 196259 • Letter: 1
Question
1. Which of the following answers is (are) true about size exclusion chromatography?
a. large proteins elute first
b. small proteins interact more with the column
c. proteins larger than the cutoff size do not elute
d. will be used to purify GFP
2. Which of the following is (are) not true about ion exchange chromotagraphy?
a. positively charged proteins elute first
b. negatively charged proteins elute first
c. uncharged proteins elute first
d. hydrophobic proteins elute first
3. The use of an antibody to capture a specific target protein best describes ............ chromatography.
4. Which of the following is (are) true about hydrophobic interaction chromatograpgy?
a. will be used to purify GFP
b. low salt buffer elutes captured proteins
c. the column is hydrophilic and the buffer is hydrophobic
d. the column is uncharged
5. To achieve the goal of isolating..................... from the bacteria, the cells must first be lysed.
a.fluorescent protein
b. pGLO
c. plasmid
d.AraC protein
Lysis will be performed using...........................
a. lysozme
b. protease
c. catalase
d. hydrochloric acid
and...................
a.freezing-thawing
b. boiling
c. vortexing
d. sonicating
6. The bacteria that you previously transformed will be cultured overnight in nutrient broth. To grow only flourescent bacteria, the nutrient broth must provide?
a. arabinose
b. AraC
c. ampicilin
d. beta lactamase
e. pGLO
f. arabinose
7. If bacteria growing on the LB/amp plate were streaked on LB/amp/ara plates then?
a. they would fluoresce immediately
b. they would not flouresce because they do not have plasmid
c. they would die
d. they would not flouresce but their daughter cells would flouresce
e. they would flouresce once gene expression occured.
Explanation / Answer
1. All are correct.
2. C is wrong remaining all correct
3. Affinity chromatography
4. A is correct
5. lysozyme
6. Ampicillin
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