L. What is the correct order of enzyme action during DNA replication? Number the
ID: 194603 • Letter: L
Question
L. What is the correct order of enzyme action during DNA replication? Number the steps from 1 to 7 Synthesis of RNA primers (priming) Helicase/topoisomerase activity Ligation 3Single-stranded binding proteins bind and SDNA polymerization/proofreading prevent reannealing 5'-3' exonuclease activity/DNA polymerizationBinding to origin of replication (AT rich sequence) Il. Using the genetic code table below, please answer the following questions: SECOND LETTER H A a) Transcribe and translate the following DNA. Label any nudeic acids with their 5' and 3' ends DNA 3TACATAACCATT 5 b) Using the DNA sequence above, write a new DNA sequence from 3' to 5'that incorporates a silent mutation c) Using the DNA sequence above, write a new DNA sequence from 3'to 5' that incorporates a nonsense mutation d) Using the DNA sequence above, write a new DNA sequence from3'to 5'that incorporates a missense mutation in the second amino acid. Created by Dr.Susan A. Holechek for BIO 340(2018)Explanation / Answer
Que I
Ans:
The correct order of enzyme action during DNA replication is:
1. Binding to origin of replication ( AT rich sequence).
2. Helicase/ topoisomerase activity.
3. Single stranded binding proteins bind and prevent reannealing.
4. Synthesis of RNA primers (priming).
5. DNA polymerization/ proofreading.
6. 5'-3' exonuclease activity/ DNA polymerization.
7. Ligation.
Explanation:
- DNA replication begins at specific site termed as origin of replication, which has a specific sequence that can be recognized by initiator proteins called DnaA.
- Helicase is recruited to the site for unwinding the helices into single strand.
- Single strand binding proteins bind to the unwound region and prevent them for annealing.
- DNA primase synthesizes a short stretch of RNA onto the existing DNA strand.
- DNA polymerases add new nucleotides only to the 3' end of an existing strand.
- 5'-3' exonuclease activity remove RNA primer.
- Ligation.
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