A) what chemicals break peptide bonds in Respretin? Select all that apply. B) wh

Question

A) what chemicals break peptide bonds in Respretin? Select all that apply.
B) what chemicals cause phosphorylation of Respretin? Select all that apply.
C) which chemicals have no observable effect on Respretin in this experiment? Select all that apply. History Bookmarks Develop Window Help usi.blackboard.com Take Test Chapter 4 Assignment-C Use the information below to answer Questions 11-13 In your lab, you study a protein caled Respretin, which has a molecular mass of about 50 kilodaltons (kDa). You are interested in how five different chemicals aect Resprein. You hypothesize that some chemicals break peptide bonds in Respretn, and others you think cause phosphorylation of Respretin. To test your hypothesis, you grow up 5 dishes of cells and you treat each one with either Chemical A, B, C, D, or E for 24 hours and then perform a Western blot using antibodies against Respretin. You receive the Western blot shown below. Answer the questions concerning the Western blot image Chemicals 245 180 100 75 -63 48 35 25 20 Click YES below to indicate that you understand the instructions Chek Suce and Subenit to stee and sbtChek Sve A Ansns to sure all ansrers Save All A MacBook Air

Explanation / Answer

1.

Breaking peptide bonds in a protein would produce smaller peptides.

We can observe smaller fragments in lane A and D.

So, chemicals A and D break peptide bonds in the given protein.

2.

Phosphorylation is a post translational modification that can affect the mobility of a protein.

Multiple phosphorylations can increase the size of a protein slightly.

We can observe supershifted protein movement in lanes C and F.

So, chemicals C and F may phosphorylate the protein.

3.

Protein is migrating at the same distance in both the control lane and lane B.

So, chemical B has no effect on the given protein.

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