T-Mobile 9:21 AM Quiz 2: Attempt 1 Question 1 1 out of 1 points You have a gene
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T-Mobile 9:21 AM Quiz 2: Attempt 1 Question 1 1 out of 1 points You have a gene that can undergo alternative mRNA splicing. You'd like to determine the mRNA form that has been favored when cells are treated with a particular compound. For this, would you use genomic DNA or cDNA to amplify your gene from treated cells? Question 2 0 out of 1 points "You are working in an agricultural firm that has designed a new gene that will improve pest resistance in plants. You add the gene to plant cells using a vector that you have designed. Although you are able to transform the vector successfully into plant cells, when the plant cells divide you are alarmed to find that vector remains present in only one daughter cell of any dividing pair of cells. What characteristic of the vector do you think you missed in the design of the vector?Explanation / Answer
1. cDNA is used to amplify the gene from treated cells. Because in alternative splicing Different exon joined together and produces a mRNA. Eg. If we have 4 exon means Exon 1, Exon 2, Exon 3 and Exon 4. In this Exon 1 and Exon 3 join together and do their function for specific compound. So we have to see onlt this mRNA codon. So we have to take cDNA from this mRNA for the amplification not the whole genome DNA.
2. Ori site is missing. Ori site is important for replication orgin. WIth out this no replication happen in the vector. So, only one copy is transfered to one cell and other cell is not have any vector
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