. When doing DNA isolation, what is the purpose to add BNase A? A To release RNA
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Question
. When doing DNA isolation, what is the purpose to add BNase A? A To release RNA from cells B Todgest RNA C Tosynthesize RNA . For gene amplfication using the PCR technique, A. Two primers are needed, and their sequence designs are customized based on the gene of interest. B One primerls needed, and its sequence design is austomized based on the gene of interest C Twoprimers are needed, and are commercially available because all genes use the same primens D. One primeris needed, and is commercally available because all genes use the same primer 4. For the microbiota project, the PCR reaction that amplifies 16S IRNA gene would result in A. A miture of various 16S IRNA genes from several strains of bacteria. B Asingle 16S IBNA gene from one bacterial strain. 5. A DNA doning project involves a few experiments, which one below illustrates the right sequence of activities? A. Plasmid isolation followed by ligation and then transformation B Transformation followed by plasmid isolation and then ligation C Ligation followed by transformation and then plasmid isolation 6. Which of the component(s) below are used in the cloning procedure illustrated in the lab manual part 2 for the microbiota project A. inearized pMiniT 20 Vector B. PCR product insert C. Ligase enzyme in dloning Mibxes 1 and2 D. All of the above 7. The baterial host cells used in PCR cloning kit is: A. Escherichia coll B. Solmonella enterice Helicobacter pylori To prepare a 1% agarose gel, what would be the amount of agarose that you want to use when preparing the so mL gel solution? A. 1g B. 01g C 5g D. 058Explanation / Answer
Answer :
1. A. To release RNA from cells.
RNase A or Ribonuclease A is a endoribonuclease purified from bovine pancrease. It is an important enzyme for the removal of RNA for RNA free DNA purification reaction such as Plasmid DNA purification or genomic DNA purification. RNase A specifically hydrolyze the RNA 3' of pyrimidine residues and cleaves the phosphodiester linkage to the adjacent nucleotide. RNase does not require any co-factor for activity.
2. A. Two primers are needed, and their sequence designs are customized based on the gene of interest.
PCR uses short template of single-stranded DNA and two primer that flank the target region, nucleotide and thermostable DNA polymerase to amplify the specific region of DNA.
3. B. A mixture of various 16s rRNA genes from several strains of bacteria.
4. A. Plasmid isolation followed by ligation and then transformation.
Isolation of DNA : The two basic steps - the gene of our interest & the vector.
Ligation : An enzyme called DNA ligase is used as molecular glue to join the gene of interest and the Vector (Recombinant DNA)
Transformation : The host cell which have taken up the recombinant DNA are called transformed cell and the process is called Transformation.
5. D. All of the Above.
6. A. Escherichia coli.
E.coli are small in size and have high growth rate compare to other organisms. E.coli genome is the first which is completly sequenced.
7. D. 0.5 gram.
=1 g/100 ml = X g/50 ml
= (1 g) (50 ml)/(100 ml) = X g
= 0.5 g = X g
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