1. What are some reasons a cell culture technician or biomedical researcher woul
ID: 193013 • Letter: 1
Question
1. What are some reasons a cell culture technician or biomedical researcher would need to conduct a cell count? 2. What were the approximate dimensions of the cells that you counted? HeLa? RAW? Be sure to include units. 3. There are two ways you have learned to visually assess (without a hemacytometer) whether a culture needs attention, whether just fresh media or to be "split". What are these two ways? 4. Hemacytometers are designed so that the coverslip rests exactly 100 um off the surface of the slide. Express this measurement in millimeters. 5. Which cells had a higher count? Give some explanations for why you think this is so? 6. As you learned, each quadrant of a hemacytometer contains a volume of 100 nL (nanoliters). How many nL are in 1 mL? How many microliters (L) are there in each quadrant? 7. Your DMEM media is supplemented with FBS and pen/strep ("complete media". What are the purposes of these supplements? 8. Why do you think the trypan blue solution you were given was in HBSS and not water? 9. Do you think your cells in the hemacytometer were held in place or squished by the coverslip? Justify your answer 10. Cell culture is a technique used far beyond immunological research. It is used in cancer research, drug development and testing, and almost every type of cell biology research. Google "cell culture technician jobs", and find out what starting salaries are, and what industries in Orange County are looking to hire cell culture technicians. 11. Trypanosomes are a genus of parasite that causes serious diseases in many parts of the world Trypanosoma brucei causes one disease, and Trypanosoma cruzi causes another. What diseases do they cause, and which professor in the Department of Biological Science studies trypanosomes?Explanation / Answer
1) cell counting is a determination of the number of cell within a given volume of a sample. Cell counting is essential because
a) it determine exact concentration of cell within the sample so that can provide critical information on the health status of the patient . For example : low white blood cells means person can easily infected by some disease.
b) in microbiology, cell counting is used to identify behaviour of infectious virus, bacteria and other pathogens. Counting cell within control sample within a constant time interval allow the examination of growth of microorganism.
c) it is also useful in molecular biology during some experiment. Cell counting is essential to calculate volume of particular chemical or reagent that should be applied to concentration of cell in order to gain results.
d) it is used to determine cell viability. So, researcher can identify effect of some drug, poisons substance on the cells.
2) dimension of Hela cells is 20 micron is diameter.
3) Change the color of culture medium due to presence of pH indicator in the medium. When you see culture medium colour turn red to orange and yellow lead to medium change. Another ways to observe flask in microscope if flask has almost 100% confluency means its best time to subculture means spilit the cells.
7) FBS is fatal bovine serum , it is a provide growth factor for the growth of cells and pen/strep is penicillin streptomycin , a antibiotic that prevent the medium from contamination. So these are essential componant add to the DMEM medium.
4) glass coverslip placed 0.1 mm above the surface of the counting area by ground glass ridge on the either side of the vertical grooves of the H shape.
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.