3) To get the dilution you used for plating in Tube F, you made the dilutions sh

Question

3) To get the dilution you used for plating in Tube F, you made the dilutions shown (Each is the number of microliters shown transferred from the previous tube into 1 mL total in the next tube. This means 1 mL is the final volume in the tube after the dilution is made). What is the concentration in Tube A (the first tube) in colony-forming units per mL?

your final answer!) You cou The day before, you had spread 200 uL (microliter) from TubeU 50 uL 10 uL 10 uL 10 uL 10 uL 1mL 1mL 1mL1mL 1mL 1mL 200 uL 4H0 cells A B mina units (bacteria) are there per microliter in Tube F?

Explanation / Answer

1. The colony-forming units (bacteria) are there per microliter in Tube F = 0.44

           200 ul ----------- 88 colonies

               1 ul -----------   88/ 200 = 0.44 CFU

2. colony-forming units are thereper mL in Tube F = 440

       0.2 ml ----------- 88 colonies

         1 ml ------------- 88 / 0.2 = 440 colonies

3. CFU /ml in A tibe = [50 ul / 1000 ul] X [10 / 1000] X [10 / 1000] X [10 / 1000] X [10 / 1000] X [200 / 1000]

                              = 1 / 106

   CFU /ml = 88 / 1 / 106

CFU /ml = 88 X 106

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