Based on the following data from a three point testcross: 1. Determine the gene

Question

Based on the following data from a three point testcross: 1. Determine the gene order and phase for the genes A, B and C. 2. Determine the distance between genes A, B and C. 3. Draw a linkage map with the correct gene order, allele combinations on each chromatid and distance between the three genes. For this exercise, phenotypes with capital letters are dominant and phenotypes in lower case letters are recessive. For example, "phenotype A" mean the dominant phenotype for the gene and "phenotype a" means the recessive phenotype for that gene. Show all your calculations. Remember that the three genes can be in any order. Remember that the dominant alleles do not necessarily need to all be on the same chromosome in the triple heterozygote parent. Phenotypes N 309 305 51 54 3 4 56 61 Total843 aTB Atb ATb atB atb ATB AtB aTb 1. This assignment may be typed or handwritten. 2. Turn in a hard copy of this assignment to next week's activity section. This assignment does not need to be uploaded in TurnltIn. For full credit, the hard copy must be turned in by the beginning of next week's activity section

Explanation / Answer

Gene order = TaB and alleles are in Trans form

Distance between T and a

= (56+61) + (3+4)/843

= 14.7 mu

Distance between a and B

(51+54) + (3+4)/843

105+7/843

112/843

13.2 mu

Gene Map:

T----------------14.7mu----------------a-------------13.2 mu----------B

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