4) Which of the following techniques involves hybridizing a cDNA sample to a chi

Question

4) Which of the following techniques involves hybridizing a cDNA sample to a chip containing thousands of single-stranded DNA sequences, allowing one to study the expression of thousands of genes simultaneously? (4 pts) A) PCR B) Southern blot C) FISH D) Agarose gel electrophoresis E) DNA microarray 5) In a recombinant DNA experiment, which enzyme is used to join together DNA fragments by forming phosphodiester bonds between nucleotides? Recall that this same enzyme joins together Okazaki fragments on the lagging strand during DNA replication. (4 pts) A) DNA primase B) DNA polymerase C) DNA helicase D) DNA ligase E) Reverse transcriptase 6) Ais a single-stranded DNA molecule labeled by a radioactive or fluorescent compound that is complementary to a specific sequence of DNA.Such pieces of DNA are used to identify and study cloned genes in hybridization experiments. (4 pts) A) polypeptide B) promoter C) vector D) probe E) plasm 7) A consists of cloned DNA fragments for all expressed genes in a particular tissue. (4 pts) A) genomic DNA library B) cDNA library C) PCR library D) Guggenheim library E) shotgun library

Explanation / Answer

4.DNA micro array is a technique in which cDNA is hybridized to a chip that contains thousands of single stranded DNA sequences. This technique enables one to study expression of thousands of genes simultaneously.

5.DNA ligase is the enzyme that is used to join different DNA fragments through phosphodiester bonds in Recombinant DNA technology. Same enzyme is used by cells in vivo for ligating Okazaki fragments on lagging strand of DNA in replication.

6. A DNA probe is a radiolabelled or fluorescent single stranded fragment of known sequence of DNA that is complementary to some specific sequence of genomic DNA. It is used in hybridization techniques like Southern blotting, or other molecular techniques for identifying genes of interest.

7. cDNA library means complementary DNA that is formed from mRNA by reverse trancriptase enzyme. Therefore, a cDNA library consists of cloned DNA fragments for all expressed genes in a particular tissue.

Related Questions

Navigate

• Browse (All)
• Browse 4
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.