4) Which of the following techniques involves hybridizing a cDNA sample to a chi
ID: 192324 • Letter: 4
Question
4) Which of the following techniques involves hybridizing a cDNA sample to a chip containing thousands of single-stranded DNA sequences, allowing one to study the expression of thousands of genes simultaneously? (4 pts) A) PCR B) Southern blot C) FISH D) Agarose gel electrophoresis E) DNA microarray 5) In a recombinant DNA experiment, which enzyme is used to join together DNA fragments by forming phosphodiester bonds between nucleotides? Recall that this same enzyme joins together Okazaki fragments on the lagging strand during DNA replication. (4 pts) A) DNA primase B) DNA polymerase C) DNA helicase D) DNA ligase E) Reverse transcriptase 6) Ais a single-stranded DNA molecule labeled by a radioactive or fluorescent compound that is complementary to a specific sequence of DNA.Such pieces of DNA are used to identify and study cloned genes in hybridization experiments. (4 pts) A) polypeptide B) promoter C) vector D) probe E) plasm 7) A consists of cloned DNA fragments for all expressed genes in a particular tissue. (4 pts) A) genomic DNA library B) cDNA library C) PCR library D) Guggenheim library E) shotgun libraryExplanation / Answer
4) PCR is a technique used to amplify a single gene using single stranded primers which in the PCR mix, bind to the DNA. There is no chip involved.
Southern blotting is a hybridization technique in which the DNA probes bind to the specific DNA and helps in the detection. There is no chip involved.
Fluorescent-in situ hybridization (FISH) is a technique which uses the fluorescent probes cytogenetically which binds to specific sequences on the basis of complementarity.
Agarose gel electrophoresis is a technique in which Nucleic acids migrate on the agarose gels under the effect of electricity.
Hence, A, B, C and D are incorrect.
DNA microarray is a culmination of single stranded DNA that are attached to solid chip. The mRNA if produced in the cell will bind to the DNA template and will give the signal which will indicate the expression levels.
Hence E is correct.
5) DNA primase is the enzyme that synthesize RNA primer for the synthesis of the complementary DNA strands during DNA replication. DNA polymerase is the enzyme which elongates the RNA primer by adding the nucleotides. DNA helicase is the enzyme which uses the energy of ATP to unwind the DNA. Reverse transcriptase is the enzyme that transcribe mRNA into the DNA such as in viruses.
Hence, A, B, C and E are incorrect.
DNA ligase is the enzyme that join DNA fragments by sealing the nick. They do so by forming phosphodiester bonds.
Hence, D is correct.
6) Polypeptide in which multiple peptides are attached. The peptide are the chains of the amino acids. Promoters are the region in the gene which are the initiation site of the transcription. Vector are the DNA fragment that act as a carrying vehicle for an external DNA fragment and carries it into a host organism. Plasm also does not used in hybridization experiments.
Hence, A, B, C and E are incorrect.
Probe are the single stranded DNA molecules that are tagged with radioactive molecule or fluorescent tag and used to detect specific DNA sequence.
Hence, D is correct.
7) cDNA is the collection of cloned genes that are expressed into the proteins. Hence, B is correct.
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