8) Imagine you wanted to use a human genomic DNA library to clone the human gene
ID: 192325 • Letter: 8
Question
8) Imagine you wanted to use a human genomic DNA library to clone the human gene for insulin. You will be using the rat insulin gene sequence as your DNA probe. pts) 1. Use autoradiography to identify colonies containing DNA that hybridized to the probe 2. Grow transformed cells on media with antibiotics and X-gal for blue-white screening 3. Ligate genomic DNA and vector DNA 4. Cut genomic DNA and vector DNA with restriction enzymes 5. Hybridize library DNA with labeled probe for the rat insulin gene 6. Transform bacteria with recombinant plasmid Circle the correct choice below. A)2. 3, 6, 5,4,1 B)1,2,5,6,4,3 C) 4,3, 6, 2,5,1 D) 4, 3, 5, 2, 6,1 E) 2, 6, 5, 3, 1, 4 9) Which of the following might lead to the generation of several incorrect PCR products? (4 points) A) An annealing temperature that is too low B) An annealing temperature that is too high C) Performing 30 cycles of PCR D) Using the correct primers E) Including an extension step 10) Define upstream and downstream processing when producing a protein. (8 pts)Explanation / Answer
8. The answer is
C) 4, 3, 6, 2, 5, 1
4. Cut the genomic DNA vector DNA with restriction enzymes
3. Ligate genomic DNA and vector DNA
6. Transform bacteria with recombinant plasmid
2. Grow transformed cells on media with antibiotics and X gal for the blue white screening
5. Hybridize library DNA with labelled probe for the rat insulin gene
1. Use autoradiography to identify colonies contaning DNA that hybridized to the probe.
9. The answer is
A. An annelaning temperature that is too low...it leads to binds at non specific regions and prodiveds several incorrect PCR products.
ACCORDING TO CHEGG GUIDELINES WE HAVE TO ANSWER ONE QUESTION AT A TIME. POST THE REST AS SPERATE QUESTIONS, THEN I CAN HELP YOU.
Related Questions
drjack9650@gmail.com
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.