BSA DILUTIONS Set up Bovine Serum Albumin dilutions (prepared in 0.01% SDS) to p
ID: 192297 • Letter: B
Question
BSA DILUTIONS
Set up Bovine Serum Albumin dilutions (prepared in 0.01% SDS) to provide a standard curve for future lab experiments. Each group will be given 1 mL ampoules of 2 mg/ml BSA. We will have a few extra ampoules in case of accidents. Make your dilutions in 1.5 mL eppendorf tubes.
Final BSA Concentration
Concentration of BSA Stock
Desired Volume
Volume of Stock (to be added)
Volume of 0.01% SDS (to be added)
5µg/100µL
100µL
10µg/100µL
100uL
20ug/100uL
100uL
30ug/100uL
100uL
40ug/100uL
100uL
50ug/100uL
100uL
60ug/100uL
100uL
70ug/100uL
100uL
Final BSA Concentration
Concentration of BSA Stock
Desired Volume
Volume of Stock (to be added)
Volume of 0.01% SDS (to be added)
5µg/100µL
100µL
10µg/100µL
100uL
20ug/100uL
100uL
30ug/100uL
100uL
40ug/100uL
100uL
50ug/100uL
100uL
60ug/100uL
100uL
70ug/100uL
100uL
Explanation / Answer
Stock BSA solution = 2mg/ml
to make 70 g / 100 l
2000 g BSA presents in 1000 l
70 g BSA presents in 1000 X 70 / 2000 = 35 l
thus we need 35 l BSA stock solution and ( 100-35) l= 65 l of 0.01% SDS.
The other concentation were also calculated like this.
Fnal BSA Concentration
Concentration of BSA Stock
Desired Volume
Volume of Stock (to be added)
Volume of 0.01% SDS (to be added)
5µg/100µL
2 mg/ml
100 µL
2.5µL
97.5µL
10µg/100µL
2 mg/ml
100 µL
5µL
95µL
20µg/100µL
2 mg/ml
100 µL
10µL
90µL
30µg/100µL
2 mg/ml
100 µL
15µL
85µL
40µg/100µL
2 mg/ml
100 µL
20µL
80µL
50µg/100µL
2 mg/ml
100 µL
25µL
75µL
60µg/100µL
2 mg/ml
100 µL
30µL
70µL
70µg/100µL
2 mg/m
100 µL
35 µL
65 µL
Fnal BSA Concentration
Concentration of BSA Stock
Desired Volume
Volume of Stock (to be added)
Volume of 0.01% SDS (to be added)
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