part d please 1. Suppose that someday a Martian bacterium is recovered. After tr

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part d please

1. Suppose that someday a Martian bacterium is recovered. After treatment with radiation, some colonies of this bacterium lose their characteristic green tinge (the mutant colories are white). When wild type Martian bacterium (green-tinged) are broken open and the resulting cell extract is O used to treat the mutant (white) bacteria, some of the mutant bacteria reacquire the green tinge of wild type Martian bacteria. To test the nature of Martian genetic material, separate portions of the cell extract are treatecd with DNase, RNase, proteinase K, and glycosidase, which are enzymes that hydrolyze DNA, RNA, protein, still cause reversion of the white Martian bacteria to green. Cell extract treated with either RNase or glycosidase was unable to cause reversion of white bacteria to green. a. What conclusions can be drawn from these experiments? Analysis of the lot of glycosidase used in the initial experiments revealed the presence of small amounts of proteins other than glycosidase. The treatment with glycosidase was repeated with RNasin included; RNasin is an inhibitor of RNase. b. What was the purpose of this last experiment? c. What would be your conclusion if the inclusion of RNasin in the glycosidase treatment restored the ability of the treated cell extract to cause reversion from white to green? d. What would be your conclusion if the cell extract incubated with glycosidase in the presence of RNasin still was incapable of causing reversion from white to green bacteria? Be precise in your language. For example, DNA and RNA do not revert-bacteria do. I suggest You do not need to hand in this diagram; just use it to help your understanding of the experiments

Explanation / Answer

If the cell extract incubated with glycosidase in the presence of RNasin still was incapable of causing reversion, it suggests that the genetic material is carbohydrate.

RNasein inhibits RNase. Glycosidase cleaves bonds between monosaccharides.

So, if the presence of glycosidase is preventing reversions, it means that carbohydrates (Preferably polysaccharides) are the genetic material.

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