Refer to the hemocytometer drawing. The cells numbers refer to the total cell co

Question

Refer to the hemocytometer drawing. The cells numbers refer to the total cell count in each of the numbered large squares. If the sample is UNDILUTED and UNSTAINED. What is the cell density. Show work    A) Square 1: 125 B) Square 2: 135 C) Square 3: 130 easin; ains basic cellular componens urucn asthe 2. Refer to the hemocytometer drawing in Figure 1. The cell numbers refer to the total cell count in each of the umbered large squares. If the sample is undiluted and unstained, what is the cell density of the sample? Show how you figured out your answer, and include units) Square #1: 125 #2: 135 #3: 130 #4: 135 3 mm Figure 1 Using the same cell counts for squares 1-4 as in Question #2, calculate the cell density for 1 of blood which was uted in 99 saline solution, then mixed with 100 methylene blue stain before counting. Show how you figured out

Explanation / Answer

The cell density can be calculated by the formula ;

Cell density = Average cells per small square * the dilution factor / volume of the small square

Average cells per small square = (125 + 135 +130 +135)/4 = 131.25

Dilution factor would be 1 here since undiluted sample

Volume of small square = 1mm3 (as depicted in the figure) = 0.001ml

Cell density = 131.25*1/0.001ml = 131250 cells /ml

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