Ps 3 tor Canwas (1Word HILE HOME INSER DESIGN PAGE LAYOUT REHERENYES MAILINGS RE

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Ps 3 tor Canwas (1Word HILE HOME INSER DESIGN PAGE LAYOUT REHERENYES MAILINGS REVIEWVIEW Cut AaBbCcD AaßbCeD AaßbCeD AaßbCeD AaBbCcD AaBbCcl Paste. Cnpy B , u, ke x, x' A,. . :EE , ,n· 11N r nal 1N0 Spac Replace Select Heading 1 Heading 2 Title Subtitle Subtle Em Emphasis Intense E.. Strong Ouote Format Painter sly es Question 2. (9 pts). The tollowing NA sequence has been used as template tor transcnptian originating the mRNA that was translated to produce the corresponding polypeptide as follows DNA RNA CAUGSGCUGGAA GAUUAGALU (> 3' eebpeptide N- Met Lys Asp C Point mutations have ben made to the original DNA template (indicated in bold ed color) In each case, identify the type of point mutation with respect to the ch nge in the sequence of bases in the DNA template and the translated polypeptie (use ALL necessary terms to describe the mutation type). Polnts of 20. a) (2 pts) DNA What type ot point mutation was macde in the UNA template above'? Will that point mutation affect the polypeptide translated from the mRNA transcribed from the DNA emplalc? EXPLAIN What type ot point mutation was macde in the UNA template above'? Will that point mutation atfect the polypeptide translated from the mRNA transcriped from the DNA template? EXPLAIN What type ot point mutation was macde in the UNA template above'? Will that point mutation atfect the polypeptide translated from the mRNA transcriped from the DNA template? EXPLAIN d) (3 pts) You will now introduce a TRANSVERSION SILENT point mutation to the original DNA rriulcculU al lhc bUgirir'ing Ur this queslion (in blue). Wrilc the ENTIRE DOUBLE STRANDED DNA molecule containing that mulatiori, the RNA traiscribed fromI il and the resuling 11:05 AM Type here to search

Explanation / Answer

A) the type of mutation is SUBSTITUTION in thich nucleotide C is replaced by T. YES, There will be effect on the poly peptide sequence as the codon in which this substitution took place was UGG to UGA in RNA and UGA is a stop codon so the transcription would stop right here.

B) this mutation is also substitution of G with A. change in codon would be from GGC to GGT (in RNA) and both of the codon code for glycine so there would be no effect on polypeptide sequence.

C) this type of mutation is insertion where A is inserted b/w C and T. this would induce a frame shift that result in formation of codon TAA instead of normal AAG. TAA is stop codon so again the transcription would stop here only.

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