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3 A-:1 aa 2. The following are dihybrid testcross data. Do these data approximat

ID: 190634 • Letter: 3

Question

3 A-:1 aa 2. The following are dihybrid testcross data. Do these data approximate the ratio one for independent assortment? Complete the table, calculate the , and answer the questions related to your calculations. Phenotype o O-E 4-bh 61 aaB aabb Totals 551 degrees of freedom a. In interpreting this ' value, you have b. In this case, do you accept/reject the hypothesis that these data approximate the dihybrid test cross ratio with independent assortment? c. What is the probability that the deviations are due to chance alone? d. Complete the following table for the data given in this problem. Note that the data in this prob- lem are dihybrid testcross data. Now, determine whether cach gene pair is behaving individu- ally as you would expect in a monohybrid testcross. Each trait considered individually should be expected to approximate a 1:1 ratio as a consequence of Mendel's law of segregation. Accept or reject hypothesis Hypothesis value P valuc

Explanation / Answer

Null hypothesis: The observed values are not deviating from the mendilian dihybrid testcross ratio i.e. 1:1:1:1.

Test static – Chisquare test:

Category

A_B_

A_bb

aaB_

aabb

Total

Observed values

102

61

71

97

331

Exptected Ratio

1

1

1

1

4

Exprected Values

82.75

82.75

82.75

82.75

Deviation

19.25

-21.75

-11.75

14.25

D^2

370.5625

473.0625

138.0625

203.0625

D^2/E

4.478097

5.716767

1.668429

2.453927

14.31722

X^2

14.31722

Degrees of freedom

4

-

1

3

Inference: The calculate chisquare value i.e. 14.31 is greater than the table value i.e. 7.82 at 3 DF and 0.05 probability. Hence the null hypothesis is rejected.

Category

A_B_

A_bb

aaB_

aabb

Total

Observed values

102

61

71

97

331

Exptected Ratio

1

1

1

1

4

Exprected Values

82.75

82.75

82.75

82.75

Deviation

19.25

-21.75

-11.75

14.25

D^2

370.5625

473.0625

138.0625

203.0625

D^2/E

4.478097

5.716767

1.668429

2.453927

14.31722

X^2

14.31722

Degrees of freedom

4

-

1

3