I\'m having trouble with the sample size from this article. What is the sample s

Question

I'm having trouble with the sample size from this article. What is the sample size? Thank you.

Summary

An estimated 80 million birds are killed by colliding with vehicles on U. S. roads each year [1, and millions more die annually in Europe [2 and elsewhere. Losses to vehicles are a serious problem for which various changes in roadway design and maintenance have been proposed [3. Yet, given the magnitude of the mortality reported for some species [4, we might expect natural selection to favor individuals that either learn to avoid cars or that have other traits making them less likely to collide with vehicles. If so, the frequency of road kill should decline over time. No information is available for any species on whether the extent of road-associated mortality has changed [2. During a 30-year study on social behavior and coloniality of cliff swallows (Petrochelidon pyrrhonota) in southwestern Nebraska, we found that the frequency of road-killed swallows declined sharply over the 30 years following the birds’ occupancy of roadside nesting sites and that birds killed on roads had longer wings than the population at large.

Main Text

We have worked on cliff swallows since 1982 in southwestern Nebraska, centered in Keith County at the Cedar Point Biological Station (41°12.591’ N, 101°38.969’ W), where colonially nesting cliff swallows attach their gourd-shaped mud nests in clusters on a vertical wall underneath a horizontal overhang. The birds use primarily highway bridges, overpasses and box-shaped concrete culverts underneath roads or railroad tracks as colony sites [5.

As we traveled among colonies daily in the course of our research, we stopped for each road-killed cliff swallow. We made the same effort to search for road kills and drove the same roads each year. We based our count of road kill retrospectively on the number of specimens prepared as skins and assume that the number salvageable provides a relative measure of mortality among years. We compared road kills to a sample of cliff swallows accidentally killed during mist-netting in the same years. The net fatalities (hereafter considered ‘the population at large’) did not differ from living birds caught at the same time (Supplemental information).

The number of salvageable specimens each year declined significantly from 1983–2012 (Figure 1A). This result could not be explained by concurrent decreases in the cliff swallow population size around roads, because the population increased over the 30-year period (Figure 1A). The decline in road kills also could not be related to increases in the number of avian scavengers over time, as none showed significant increases in our study area (Supplemental information). Direct information is not available for mammalian scavengers within the study area, although populations of those species associated with humans probably have not changed, given that the resident human population of Keith County varied little during the study. Also, scavengers such as skunks have declined for the state of Nebraska as a whole [6. Road-kill trends did not result from reduced vehicle traffic volume over time, which either did not change significantly or increased, depending on the metric used (Supplemental information). Sport-utility vehicles, which have probably increased during our study and offer a greater surface area for collision (relative to sedans that were more common in the 1980s), might contribute to changing bird mortality. However, road kill decreased as the larger vehicles became more common. Differences in detection likelihood did not affect our results, as the total kilometers traveled by us annually did not change significantly (Supplemental information). Thus, none of the obvious factors that confound most road-kill surveys applied to our study.

Figure 1

Changes in number and wing length of road-killed cliff swallows.

Road-killed cliff swallows in southwestern Nebraska have declined with time and constitute a disproportionately greater fraction of longer-winged birds in the years following the birds’ occupancy of roadside colony sites. (A) The number of salvageable road kills (closed circles, solid line) declined significantly with time (rs = 0.93, p < 0.0001, n = 25 years) despite the population size around roads (open circles, dotted line) increasing over the study period (rs = 0.76, p < 0.0001, n = 30 years), with year being a significant predictor of the number of road kills found (F1,22 = 38.8, p < 0.0001, GLM) but not population size (F1,22 = 0.01, p = 0.93); (B) wing lengths for birds killed on roads (dark bars) versus the population at large (as represented by mist-net fatalities; shaded bars) were significantly different (F1,229 = 7.06, p = 0.007, ANCOVA); (C) mean wing length (± SE) for cliff swallows killed on roads (closed circles, solid line) increased significantly over time (rs = 0.78, p < 0.0001, n = 20 years), while that of the population at large (open circles, dotted line) decreased significantly over time (rs = 0.63, p = 0.002, n = 21); (D) total cliff swallows reported on a Breeding Bird Survey in Keith County, Nebraska, per year 1967–2011 indicate that the species began commonly encountering vehicles at about the time this study commenced. Lines indicate best-fit least-squares regression.

Wing length of road-killed cliff swallows was significantly longer than in the population at large (Figure 1B). Over time, wing lengths of cliff swallows killed on roads increasingly diverged from that of the population at large (Figure 1C). Average wing length of the population as a whole exhibited a significant long-term decline during the years of the study, whereas the opposite pattern held for the birds killed on roads (Figure 1C).

Cliff swallows now commonly nest on highway bridges, overpasses, and road culverts [5. The Breeding Bird Survey that began in our study area in 1967 (Figure 1D) showed that these birds likely began commonly encountering vehicles when they started frequently using roadside nesting sites in the early to mid-1980s at about the time our research commenced, probably in response to construction of more bridges and culverts. Our results indicate that these birds since then have become increasingly less likely to collide with cars and that road mortality is not indiscriminate. One possible explanation is that selection has favored individuals whose wing morphology allows for better escape. Longer wings have lower wing loading and do not allow as vertical a take-off as shorter, more rounded wings [7. Thus, individuals sitting on a road, as cliff swallows often do, who are able to fly upward more vertically may be better able to avoid or more effectively pivot away from an oncoming vehicle [8.

Vehicle mortality is likely to be not the only factor contributing to the decline in wing length in this population over time; severe weather events that cause selection on body morphology and changes in insect prey may also be responsible [9. Other explanations for the reduction in road kill are that swallows may learn to avoid collisions as they encounter a vehicle themselves or observe other birds flying away from vehicles or getting hit, or that risk-taking individuals have been selectively removed [10. We cannot directly evaluate these hypotheses, although if individuals are likely to avoid cars after a close encounter, we would expect younger birds to be overrepresented among the road kills, which they were not (Supplemental information). Cliff swallows do exhibit social learning in other contexts, such as by observing the foraging success of neighbors [5. Regardless of mechanism, the drop in traffic-related mortality over 30 years suggests that researchers should consider the possibility that road mortality in other species may change temporally and exert selection.

ERA] Population at large (n = 134) Road kills o ests 9 Road kills (n = 104) 40000 35000 Q 30000 18 16 O 14 CD 16 12 10 20000 15000 10000 3 4 5000 0 1984 1988 1992 1996 2000 2004 2008 2012 Year Wing length (mm) Road kills O Population at large 113 700 600 500 400 300 200 100 E 109 O 107 105 103 1984 1988 1992 1996 2000 2004 2008 2012 Year 1964 1972 1980 1988 1996 2004 2012 Year Current Biology

Explanation / Answer

After reading the whole paper and supplemental information, I notice 7 analysis of information and data were realized, during a period of almost 30 years (1983-2011):

1)Factors Potentially Affecting Road Kills: The section's information was obtained from the BBS data base, it was already analized data about the variation in size of the population of different birds species in the area of study, as well as humans' population and their pets, as they could be influencing the cliff swallows' population. No samples were taken in this section, its data from other studies only mentioned to prove that there was no statistically significant variation in all the populations that could affect cliff swallows' population during those 30 years.

2)Phenotypic Characteristics of Road Kills: In here, a sample of 104 road-killed adult cliff swallows was compared with a sample of 134 dead birds (other cause of death) from the general population during the same years. So in this section you find two sample sizes, one of 104 (n=104) individuals and the other of 134 individuals (n=134).

3)Study area: In this section the amount of nests in the area was studied to have an idea of the population of cliff swallows in general, having an stimated size of 6000 nests for the whole population in the area, calculated from the observed sample size of 2318 (n=2318)

4)Collection of Road Kill: This section only mentions if the collection of road-killed birds was the same ove the time, in terms of time spend and distance traveled during the recolections, which they conclude it was. No sample sizes for this section.

5)Vehicle Traffic Estimation : In this section as well as in section 1 they recolect already analyzed data from another study to determine if vehicle traficc chenged over the time, so no sample sizes for this section.

6)Population Size and Breeding Bird Survey:They here mentioned they summed the annual colony sizes (number of nests) for each colony site in their main study area each year, and used that as the measure of cliff swallow population size.

7)Wing Measurements, Determining Sex and Age, Designating Population at Large: The sample sizes for this section is the same as the measurements in the title were obtained from the road-killed adult cliff swallows and dead adult cliff swallows with anyother cause of death.

So in summary the only sample sizes are n=104 for road-killed adult cliff swallows, n=134 for adult cliff swallows with anyother cause of death, and n=2318 for number of nests.

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