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4. A 65-y/o man lung with sewrom eastem Euroe comes to you with a severe cough.

ID: 190045 • Letter: 4

Question

4. A 65-y/o man lung with sewrom eastem Euroe comes to you with a severe cough. His x-ay showed haziness throughout his right eastern ung with several round areas of clearing surrounded by haziness. You try a traditional culture, but there is no growth after two days. So you do an acid-fast stain on his sputum and find it is positive. (a) What bacterium caused this illness? (b) Why was there no growth after two days if there are living bacteria in the sputum? (c) What aspect of the bacteria does the acid-fast stain differentiate? (d) With a generation time of 12 hours, how long would it take for 100 cells to become a visible colony (over 1,000,000 cells)? Show your work dams oecause khy badenum has nerther gam Past-hre d lacu peptido Iyan . Tre aud-fast Stin heats 27 7

Explanation / Answer

D. The formula is: X = X0 . 2y    Here X = 1000000, X0 = 100 and y = number of generation required for 1000000 individuals.

Therefore 100 . 2y = 1000000

or 2y = 10000

y = 13. 3 (approx)

Thus the total number of generation required to obtain more than 1000000 individual is 14. Thus total time required is equals to 14 X 12 = 168 hrs. (since one generation take 12 hrs).

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