Sally is a laboratory researcher sludying human genetics A collaborator at a hos

Question

Sally is a laboratory researcher sludying human genetics A collaborator at a hospitai sends her tiopsy canpies from s of interest. When she runs the reactions on an agarose gel she sees no tands. Which of the tolowing codld eplain he 1: In addition The DNA b negatively charged, so tan toward the Dosavey darged entude Sany forgot to add dNIPs to her readin The DNA is positively charged, so t ran toward he negator cnarped ouchode your notes on pyou may firndithegfultorevow your BOLAS,, notes on ge'cloctopnoresa K Saly accidentaly added a bufer contarang Mg2·ons to hereacton Sally tergot to start the PCR machine Contamination Sally forgot to add loading dye to her sampies before running the g & The Taq DNA potymerase was denalured by first 95P C se, so it was nactue tor the rest of the neaion when Sanys PCR had reached 5 cycles, there aas a ppoage in the uldia asing the thernal cydler to tum o Saly forgot to add primers to her reaction Sally sneezed in her PCR tubes Soly accidentally added a second par of prmera o her isaction in ain er ielended psmes The DNA os ar come from a human so re prmes dd nol bend the lempate R Saay designed her primers inconnny

Explanation / Answer

This are the reasons she does not got any bands on the gel.

1. Sally forget to add dNTPs to her reaction.
2. Sally forgot to start the PCR machine
3. Sally forgot to add loading dye to her sample before running the gel. - Loading buffer is added to a DNA sample to give it color to the naked eye. .
4. Sally forgot to add primers to her reaction
5. Sally designed her primer incorrectly.

Mg2+ important in the PCR reaction. It act as a cofactor. So it won't comes.

Related Questions

Navigate

• Browse (All)
• Browse S
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.