12) A peptide was treated with protease enzymes and cyanogen bromide followed by

Question

12) A peptide was treated with protease enzymes and cyanogen bromide followed by complete hydrolysis of the resulting products. Based upon the following data, determine the sequence of the original peptide Chymotrypsin gave two peptides containing the following amino acids Leu, Pro & Asp, Gln, Arg, Met, Tyr, Val Cyanogen bromide gave two peptides containing the following amino acids: Asp, Met &Gln;, Leu, Arg, Tyr, Pro, Val Trypsin gave two peptides containing the following amino acids GIn, Leu, Tyr, Pro &Asp;, Arg, Met, Val

Explanation / Answer

ANSWER: As cleavage locations of trypsin, chymotrypsin and cyanogen bromide are all at the carboxyl side of the residues so the probable sequence is

Leu-Pro-Tyr-Gln-Arg-Val-Met-Asp

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