12 of 1 Part A What is the %GC content? 5-TTGAAAATATTTCCCATTGCC-3\' Round your a

Question

12 of 1 Part A What is the %GC content? 5-TTGAAAATATTTCCCATTGCC-3' Round your answer to the nearest whole number. ?? Submit Request Answer Part B What is the annealing temperature for this oligonucleotide? 5-TTGAAAATATTTCCCATTGCC-3 Use your answer from Part A (%GC content) and the length of the oligonucleotide primer (N) to compute the melting temperature (Tm). Remember that you must subtract 5 degrees from the Tm value to calculate the annealing temperature. Round your answer to one decimal place (example: 65.1 or 70.0). Submit Request Answer

Explanation / Answer

%GC content is the number of GC bases present in the given DNA sequence.
Given sequence is 5' TTGAAAATATTTCCCATTGCC-3'
%GC content is 33.33%
Answer is 33%

Based on number of bases and %GC content, the annealing temperature comes out to be
46.5 C. So annealing tempearture would be 41.5C

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