I know the answer is arg-met-pro-, but why? Master Plate REPLICA PLATE arg met m

Question

I know the answer is arg-met-pro-, but why?

Master Plate REPLICA PLATE arg met met +pro pro +arg arg + met + pro Approximately 108 E. coli cells of a mutant strain are plated on complete media forming a bacterial lawn (the master plate). Replica plates are prepared containing minimal medium supplemented with various different combinations of the amino acids arginine, methionine and proline as shown above. The genotype of colony 1 is: A arg met+ pro* B arg met pro C arg met pro D arg met pro E arg met pro Video 9 12

Explanation / Answer

From the arg+met supplemented minimal media, we can know that the colony 1 does not formed, though it is supplemented with arg and met. So we can conclude that the colony 1 require pro supplement, because it does not contain pro coding gene. So it is pro-.

From the met+pro supplemented minimal media, we can know that the colony 1 forms, even in the absence of arg. So we can conclude that the colony 1 contains arg coding gene. So it is agr+.

From the pro+arg supplementes minimal media, we can know that the colony 1 does not formed, though it is supplemented with arg and pro. So we can conclude that the colony 1 require met supplement, because it does not contain met coding gene. So it is met-.

arg+pro+met supplemented media also confirms that the colony 1 is formd when it is provided with the met and pro, it lacks.

From all these conclusion we can say that the colony 1 forming bacteria is arg+ met- pro- (Option D)

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