Based off of : https://pdfs.semanticscholar.org/f958/6d44ab895cce19e7a6769f5517b

Question

Based off of : https://pdfs.semanticscholar.org/f958/6d44ab895cce19e7a6769f5517b224cbf0c2.pdf

the authors generate hybrids between two species of monkeyflowers, M. nasutus and M. guttatus, We can imagine there is a gene D with two alleles: Dis the allele from M. nasutus, and D is the allele from M. auttatus Using these symbols, what would the genotype be of a pure M. nasutus plant? Pure M. guttatus? a. F1 hybrid? (1 pt) b. What are the expected genotypes, and in what proportions, in the F2 generation? (1 pt) The authors genotypes 287 F2 plants. What are the expected numbers of genotype, given the proportions you determined in b? C1 pt) c. d. In the F2 intercross experiment, what genotypic proportions do the authors observe on a particular part of linkage group (chromosome) 11? (1 pt) Imagine that the observed numbers of F2 individuals of each genotype are: 3 DnD 144 DDm, and 140 DmD. Perform a Chi-square analysis to determine whether the authors can reject the null hypothesis of equal segregation of alleles. (2 pt) e.

Explanation / Answer

a) M. nasutus - DnDn

M. guttatus - DgDg

F1 hybrid - DnDg

b) ratio = 1 DnDn : 2 DnDg : 1 DgDg

c) total plant =287

4x = 287

x = 72 plants homozygous Dn , 72 plants homozygous Dg and 143 plants heterozygous.

d) only 1% of total genotypic proportion was observed on a particular part of linkage group

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