(21). This study compared changes in the expression of proteins in response to h
ID: 188080 • Letter: #
Question
(21). This study compared changes in the expression of proteins in response to heat stress between an Antarctic fish, Trematomus bernacchi (A) and a temperate fish, Gillichthys mirabilis (B). Trematomus bernacchi is found in the Southern Ocean where water temperatures are constantly cold at ~-1°C. G. mirabilis inhabits tide pools off the coast of California and Oregon where water temperatures are more variable. Results are shown below. Recall that two important classes of HSPs are 60 kDa and 70 kDa. . Temperature ("O B Temperaturo (°C) S 8 10 200 kDa - 116 kDa - - 100 KDa -90 kDa 97 kDa - -- 70 kDa -- 60 kDa 66 kDa - - 40 kDa (LMW) - 20 kDa (LMW) 45 kDa - 31 kDa - 21 kDa - Figure. (A) Effects of temperature on protein synthesis patterns of hepatocytes (liver cells) isolated from Trematomus bernacchii. Hepatocytes were exposed to the temperature indicated above each lane for 1 h. Proteins in cell lysates were separated using SDS-polyacrylamide gel electrophoresis. Positions of protein molecular mass standards areExplanation / Answer
Answer:
Based on the given information:
a) How does the expression of HSPs change in response to heat stress in each species
b) Does this surprise you? Why or why not? How might you explain the results?
c) What controls were used in theses experiments
d) Were the experimental conditions the same for both species? How might this have affected the results?
e) What technique could you use to detect the presence of a single heat shock protein
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