Date: Name: Module 3 Lab Assignment These questions are based on the Module 3 La
ID: 187088 • Letter: D
Question
Date: Name: Module 3 Lab Assignment These questions are based on the Module 3 Lab exercise, and the supporting information in the lab materials folder Explain why we can talk about alleles when analyzing SNPs. What is the genotype of students 1, 2, and 3 from the gel below? For each student, are they a heterozygote or a homozygote? 1. 1500 Key: 100 bp DNA ladder 1 student 1, etc. U- uncut C- cut 500 400 300 200 The picture on the left is the result of a gel electrophoresis of the DNA from 3 different students The furthest sample on the left, labeled "L" at the top, is a size standard that can be purchased so that we can have DNA of known sizes to compare our human DNA against. The picture on the right shows the sizes of this 100 bp DNA ladder, or size standard. So in the L lane on the gel on the left, the BOTTOM line is at 100 bp, the next one up is 200, the next one up is 300, etc. Use the information from the Module 3 lab exercise, especially the chart on p. 3 where you wrote in the sizes of the DNA if it cut or did not cut with Hpall, to answer this question.Explanation / Answer
Alleles are alternative copies of a gene.
They differ with each other due to the presence of SNP or indels.
Different alleles must show difference DNA sequence but their function can be similar or different.
In the given case, an SNP is responsible for the allelic difference which can be distinguished using PCR and HpaII digestion.
Assumption: WT allele can not be cleaved by Hpa II. Only mutant allele can be cleaved by Hpa II.
So, if a person is homozygous WT, he will produce a single undigested band.
If a person is a homozygous mutant, he will produce two digested bands.
If a person is heterozygous, he will produce three bands (2 digested + 1 undigested bands).
Student 1 = Heterozygous
Student 2 = Heterozygous
Student 3 = Homozygous mutant
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