Design a semester long project using RAW cells or mouse macrophages with protoco

Question

Design a semester long project using RAW cells or mouse macrophages with protocols ranging from counting cells to using flow cytometry with the use of fluorescent microspheres. Other protocols include NO measurement production by Greiss Reaction, detection of apoptosis, western blot. Can use some mice for peritoneal injections with CD11b PE. What I’m trying to say is, if you can design it I can prob execute it. Design a semester long project using RAW cells or mouse macrophages with protocols ranging from counting cells to using flow cytometry with the use of fluorescent microspheres. Other protocols include NO measurement production by Greiss Reaction, detection of apoptosis, western blot. Can use some mice for peritoneal injections with CD11b PE. What I’m trying to say is, if you can design it I can prob execute it.

Explanation / Answer

Cell lines, such as RAW cells or mouse macrophages are highly useful in vitro model for conducting various studies. These cells can be easily grown in laboratory to conduct the desired experiments. Following set of experiments can be designed using these cell for a time period of nearly 4-5 months, where the primary target is to evaluate the following:

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